抗狂犬病ウイルス核タンパク質IgY の調製 : アジュバント前投与DNA免疫法とタンパク質免疫法との比較

Abstract

Rabies is one of the zoonoses which is prevalent mainly in developing countries. Specific antibodies against the rabies virus are needed for rabies research, diagnosis, and treatments in those areas; however, these tended to be in short supply. This study compared protein immunization and DNA immunization effects for the more straightforward preparation of the anti-rabies virus Nucleoprotein IgY from chicken eggs. As the result of ELISA with diluted egg yolks, most of the protein-immunized hens and some DNA-immunized hens induced high ELISA values. Then, adjuvant injection before DNA immunization (Pre-immune stimulation) was more effective in obtaining higher ELISA values of IgY than without Pre-immune stimulation. Commonly, it takes a long time and tedious work to prepare purified protein for use as antigens. Therefore, this DNA immunization method with Pre-immune stimulation would efficiently supply specific IgY antibodies to endemic countries.