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Please use this identifier to cite or link to this item: http://hdl.handle.net/11173/3387

Title: 抗狂犬病ウイルス核タンパク質IgY の調製 : アジュバント前投与DNA免疫法とタンパク質免疫法との比較
Other Titles: Preparation of specific IgY against Rabies virus nucleoprotein : Comparative study of adjuvant pre-injected DNA immunization and protein immunization methods
Authors: 久保, 七彩
上羽, 智恵美
門間, 敬子
井上, 智
野口, 章
八田, 一
Kubo, Nanase
Ueba, Chiemi
Momma, Keiko
Inoue, Satoshi
Noguchi, Akira
Hatta, Hajime
Issue Date: 14-Jan-2022
Publisher: 京都女子大学食物学会
Abstract: Rabies is one of the zoonoses which is prevalent mainly in developing countries. Specific antibodies against the rabies virus are needed for rabies research, diagnosis, and treatments in those areas; however, these tended to be in short supply. This study compared protein immunization and DNA immunization effects for the more straightforward preparation of the anti-rabies virus Nucleoprotein IgY from chicken eggs. As the result of ELISA with diluted egg yolks, most of the protein-immunized hens and some DNA-immunized hens induced high ELISA values. Then, adjuvant injection before DNA immunization (Pre-immune stimulation) was more effective in obtaining higher ELISA values of IgY than without Pre-immune stimulation. Commonly, it takes a long time and tedious work to prepare purified protein for use as antigens. Therefore, this DNA immunization method with Pre-immune stimulation would efficiently supply specific IgY antibodies to endemic countries.
URI: http://hdl.handle.net/11173/3387
Appears in Collections:第76号(2022-01-14)

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