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第64号(2009-12-25) >

Please use this identifier to cite or link to this item: http://hdl.handle.net/11173/1756

Title: モノクローナルIgAの作製とその利用
Other Titles: Production and Application of IgA Monoclonal Antibody
Authors: 木津, 久美子
Kizu, Kumiko
廣瀬, 潤子
Hirose, Junko
山口(村上), 友貴絵
Murakami-Yamaguchi, Yukie
成田, 宏史
Narita, Hiroshi
Issue Date: 25-Dec-2009
Publisher: 京都女子大学食物学会
Abstract: We hypothesize that IgA immune complex in mother's milk functions for the prevention of food allergy through oral immune tolerance. To prove this hypothesis and to establish the allergic cure by using IgA immune complex as vaccine, a mass preparation of mouse IgA was performed. We established monoclonal antibody producing hybridomas with mesentery lymph nodes and got three kinds of monoclonal IgA. Their Light chains were all κ and the molecular weight was about 280 kDa, suggesting their dimmer form. The antigen specificity of these antibodies was unidentified, but they did not react with major food allergens. Monoclonal IgA was effectively purified with a Protein L column from the culture supernatant of the hybridoma. Ovalbumin was coupled to the monoclonal IgA with a hetero-bifunctional crosslinking reagent, Sulfosuccinimidyl 6- [3' (2-pyridyldithio) -propionamido] hexanoate. Formation of the pseudo IgA immune complex was confirmed by a sandwich type of Enzyme Linked Immunosorbent Assay with the anti-ovalbumin antibody and the anti-mouse IgA (α) antibody
URI: http://hdl.handle.net/11173/1756
Appears in Collections:第64号(2009-12-25)

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