小麦アレルギーのエピトープペプチドに対するモノクローナル抗体の作製

Abstract

Monoclonal Antibodies were raised against SQQQ(Q)PPF peptide conjugated to Keyhole Limpet Hemocyanin, which has been identified as epitope peptide of wheat allergy. Three monoclonal antibodies, mAbs 3D, 4B, and 6F were obtained by screening with the peptide conjugated to bovine serum albumin. However, these mAbs did not react with the free peptide in a competitive enzyme-linked immunosorbent assay. On western analysis, about 14 and 17kDa proteins in the wheat globulin fraction were detected with these mAbs. It is reported that wheat proteins of about 14, 16kDa were reactive with sera of patients allergic to wheat and that a wheat globulin of about 19kDa has SQQQQQG sequence. It seems likely that the mAbs obtained here recognize the 19kDa protein related to wheat allergy.