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Please use this identifier to cite or link to this item: http://hdl.handle.net/11173/3552

Title: Repeated Starvation Leads Fat Accumulation in Caenorhabditis elegans
Authors: Yamamoto, Rena
Yamamoto, Kanae
Sato, Nao
Shimojitosho, Satomi
Yamamoto, Sayuri
Sawanaga, Akari
Matsumoto, Shinya
Keywords: repeated starvation
Caenorhabditis elegans
fat accumulation
Issue Date: 31-Dec-2022
Publisher: 京都女子大学食物学会
Abstract: Our aim is to define the effect of repeated-starvation on organism using nematode Caenorhabditis elegans. Adult worms were exposed to two cycles of 6 hr starvation-18 hr feeding protocol, and fat content in worms was analyzed by Nile Red staining and biochemical quantification. The expression of genes involved in fat synthesis (pod-2, fasn-1, mboa-2, sbp-1) and fat degradation (hosl-1, lipl-4, cpt-1, cpt-2, B03003.3, F53a2.7) was also analyzed by quantitative RT-PCR. Both Nile Red staining and biochemical quantification showed that fat content in worms that experienced repeated-starvation increased. There was no obvious change in the expression of genes involved in fat synthesis, but those of genes involved in fat degradation tended to decrease, which is consistent with the increment of fat in worms experienced repeated-starvation. The lifespan, fecundity and mobility of worms that experienced repeated-starvation did not show remarkable differences compared to those of the fed worms. However, the pharyngeal pumping increased upon experiencing starvation, indicating amount of food intake increased by starvation. Our results indicate that repeated-starvation caused metabolic and nutritional effect in worms. It is often mentioned that repeating weight loss leads to weight regain, sometimes referred as “weight rebound”, and our data may also provide a molecular basis of weight regain.
URI: http://hdl.handle.net/11173/3552
Appears in Collections:第77号(2022-12-31)

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