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Please use this identifier to cite or link to this item: http://hdl.handle.net/11173/2274

Title: ヒトIgA に対するイムノクロマトグラフィーの開発
Other Titles: Development of Immunochromatography for Human IgA
Authors: 松永, 安由
Matsunaga, Ayu
木津, 久美子
Kizu, Kumiko
井上, 早姫
Inoue, Saki
浦田, 采香
Urata, Ayaka
貫洞, 美穂
Kandou, Miho
成田, 宏史
Narita, Hiroshi
Keywords: IgA欠損症
イムノクロマトグラフィー
モノクローナル抗体
Issue Date: 21-Dec-2015
Publisher: 京都女子大学食物学会
Abstract: Immunochromatography (ICG) strip test based on monoclonal antibodies (mAbs) conjugated with gold nanoparticles was developed and its application for primary screening of IgA in human saliva was evaluated. Six mAbs were established against human secretory IgA. anocolloidal gold as the detection reagent was labelled with mAb ④. MAb ⑥ was immobilized on a nitrocellulose membrane as the capture reagent to prepare the ICG strip test. After reaction of mAb ④-nanocolloidal gold probe with IgA in saliva, resulting immune complex was vertically developed, captured by mAb ⑥ immobilized on the membrane and visualized as a gold band. In the optimized investigational conditions, the ICG strip test could distinguish human secretory IgA in the range from 1 to 20 μg/mL and was sufficiently sensitive to find out incomplete IgA deficiency from salivary screening. It took only 10 minutes to accomplish a detection of salivary IgA in this assay, compared to 5 hours by sandwich ELISA.
URI: http://hdl.handle.net/11173/2274
Appears in Collections:第70号(2015-12-21)

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