モノクローナル抗体を用いた殺菌剤クロロタロニルに対する酵素免疫測定法の開発

Abstract

Chlorothalonil (tetrachloroisophthalonitrile) is a compound which has been widely used as germicide. A competitive enzyme-linked immunosorbent assay (ELISA) was developed for the detection of residual chlorothalonil in crops. Three monoclonal antibodies, mAbs 12E, 9A, and 11D were generated against bovine serum albumin-comjugated pentachlorophenol as a homologue of chlorothalonil. By using these mAbs, a direct competitive ELISA has been done. Detectable range of chlorothalonil in the ELISA was 1-3ng/ml for mAb 12E and 0.3-3ng/ml for both mAbs 11D and 9A. MAb 9A reacted with chlorothalonil 3 times stronger than another structurally related germicide fthalide, while mAb 11D reacted with fthalide 5 times stronger than chlorothalonil. The proposed ELISA with mAb 9A would be useful for convenient monitoring of residual chlorothalonil in crops.