卵管における卵黄膜外層タンパク質の分泌部位の解析

Abstract

To clarify the formation of the vitelline membrane outer layer we examined the proteins secreted from hen oviduct by using Western blotting analysis. Antibodies were elicited against vitelline membrane outer layer protein-I (VMO-I) and against the one of subunit (S 8) of ovomucim obtained from albumen. It was found that VMO-I was mostly secreted from the middle region of the infundibulum. On the contrary, S 8 was secreted from the lower region of the infundibulum over the lower region of the magnum. These findings indicated that 1) VMO-I is present in the infundibulum prior to the formation of the outer layer, 2) the chalaziferous layer is formed at the upper region in the magnum, 3) the albumen is formed at the middle region to the lower region in the magnum.