http://hdl.handle.net/11173/579 1999-12-10 2026-04-19T09:48:19Z http://hdl.handle.net/11173/1386 タイトル: マウスリンパ組織におけるレプチンレセプターの発現 著者: 井田, めぐみ; Ida, Megumi; 草信, 映子; Kusanobu, Eiko; 鈴木, 真知子; Suzuki, Machiko; 酒井, 奈美; Sakai, Nami; 白井, 能富子; Shirai, Nobuko; 永尾, 命子; Nagao, Meiko; 幟川, 智子; Noborikawa, Tomoko; 宮田, 堅司; Miyata, Kenji 抄録: The mammalian thymus involutes with age. Both the decrease of lymphocyte-accumulated region and the replacement by fat tissue begin at puberty and continue throughout life. In the mouse thymus, though no typical fat cells are found at birth, fat cells appeared at the latest 3 weeks old preceding to the atrophy of cortical and medullary regions. Physiological functions of fat cells proliferated and differentiated in the thymus after birth are unknown. As fat cells appeared in the 3-week-old mouse thymus have shown to express leptin mRNA, we examined whether leptin receptor type b (OBRL) is expressed or not in the thymus and, in comparison, in the secondary lymphoid tissues, spleen, mesenteric lymph node and Peyer' s patch. OBRL mRNA was detected in both thymus and all secondary lymphoid tissues examined by RTPCR method. Furthermore, OBRL mRNA was also detected in the isolated cells with mouse-lymphocyte separation medium from both thymus and spleen free cells washed out in saline from dissected tissues. It is suggested that fat cells differenciated as early as 3 weeks old in the thymus produce leptin and affect other thymus cells, lymphocytes probably, through OBRL. It remains to be clarified whether the leptin-OBRL interaction influences age involution in the thymus. 1999-12-09T15:00:00Z http://hdl.handle.net/11173/1383 タイトル: 高速液体クロマトグラフィーによる食品中の農薬分析 著者: 六嶋, 紀子; Rokushima, Noriko; 安部, 尚子; Abe, Naoko; 岡本, まゆみ; Okamoto, Mayumi; 近藤, 陽太郎; Kondo, Yôtaro 抄録: Reversed-phase high performance liquid chromatography (HPLC) using acetonitrile-water as a mobile phase and UV detection is performed for a rapid and simultaneous determination of 25 pesticides of different types, namely organophosphorus, organochlorine, carbamates, triazines and nitroaniline in fruits, vegetables and tea leaves. The pesticides were extracted with acetone and then partitioned with dichloromethane. Clean-up of the samples was accomplished by open-column liquid chromatography with florisil. Eleven pesticides were detected from 22 among 35 samples.The detection limits of the present method were less than 0. 001μg/g. 1999-12-09T15:00:00Z http://hdl.handle.net/11173/1384 タイトル: アスコルビン酸ステアリン酸エステルを投与したマウスにおける鉛毒性の軽減 著者: 植木, 芽美; Ueki, Megumi; 山中, 美幸; Yamanaka, Miyuki; 綿貫, 靖子; Watanuki, Yasuko; 滝本, 恭子; Takimoto, Kyoko; 松倉, 理恵; Matukura, Rie; 岡本, 麻友子; Okamoto, Mayuko; 毛利, 友美; Mohri, Tomomi; 中川, 一夫; Nakagawa, Kazuo 抄録: We aimed to evaluate roles of hepatic ascorbic acid in ddY strain mice after a single injection of lead acetate (100μmol/kg body weight, i.p.). Lead decreased glutathione content, inhibited glutathione S-transferase activity, and increased calcium content in the liver four days after the injection. These lead-induced alterations were significantly antagonized by the treatment of mice with a diet containing 1(w/w) % ascorbyl stearate ester (ASE) for three days before and four days after lead injection, whereas ascorbate content was largely elevated in the livers of animals treated with both lead and ASE. Furthermore, the production of NADPH was enhanced by not only lead injection but also ASE-feeding. These results suggest that ASE-feeding could ameliorate cell damage through the restoration of intracellular redox states. 1999-12-09T15:00:00Z http://hdl.handle.net/11173/1385 タイトル: 陰膳方式食物収集による日本人の栄養調査 : 血清脂質と栄養摂取量 著者: 寺本, 夕美子; Teramoto, Yumiko; 井上, 和佳子; Inoue, Wakako; 川本, 佳奈; Kawamoto, Kana; 三宅, 紀美子; Miyake, Kimiko; 東川, 佳絵; Higashikawa, Kae; 渡辺, 孝男; Watanabe, Takao; 松田(猪口), 尚子; Matsuda-Inoguchi, Naoko; 中塚, 晴夫; Nakatsuka, Haruo; 張, 作文; Zhang, Zuo-Wen; 池田, 正之; Ikeda, Masayuki; 新保, 愼一郎; Shimbo, Shin-ichiro 抄録: Nutrient intake was surveyed by total food duplicate method in combination with serum biochemistry among adult Japanese women. The survey was conducted twice in the same 19 sites all over Japan, once in the years around 1980 (the first survey on 199 women) and then in 1991-6 (the second survey on 379 women). The participants 19 sites were classified into four groups of those in Hokkaido, urban areas, rural areas and Okinawa by the location of residence, and relation of findings serum lipid analyses with nutrient intakes was examined. In the Hokkaido group, cholesterol and triglyceride in serum, and intake of animal fat and saturated fatty acids (SFAs) were lower in the second survey than in the first survey. In the urban group, serum cholesterol, and intakes of animal fats and SFAs were the highest among the four groups both in the first and second surveys. In contrast, serum cholesterol, and intakes of animal fats and SFAs in the rural group were lower than the levels in other groups in the first survey, but the three groups in the second survey. In Okinawa, serum cholesterol was lower than others in the second survey. Intake of fat-based energy was high, but intakes of monounsaturated fatty acids and n-6 polyunsaturated fatty acids were also high. When all subjects were combined, the serum cholesterol level was 193mg/100ml (as an arithmetic mean) in the first survey and it was 202 mg/100 ml in the second survey, and this increase was paralleled by the increase in the number of aged survey subjects. Serum triglyceride level was 111mg/100ml in the first survey, and 99 mg/dl in the second survey. The reduction may be attributable to the reduced nutrient intake among young people. Serum cholesterol correlated significantly with intake of animal fats and SFAs, but the correlation with total energy intake was significant only in the second survey. BMI correlated significantly with serum triglyceride levels in both surveys, but the correlation coefficient was smaller in the second survey than in the first survey. There was no correlation between BMI and serum cholesterol in both surveys. 1999-12-09T15:00:00Z