DSpace コレクション: 2000-12-102000-12-10http://hdl.handle.net/11173/5802024-01-05T00:21:43Z2024-01-05T00:21:43Zニワトリ卵巣での卵成熟に伴う卵黄膜内層タンパク質の変化橋本, 康代Hashimoto, Yasuyo木戸, 詔子Kido, Shokohttp://hdl.handle.net/11173/13932014-03-21T16:35:36Z2000-12-09T15:00:00Zタイトル: ニワトリ卵巣での卵成熟に伴う卵黄膜内層タンパク質の変化
著者: 橋本, 康代; Hashimoto, Yasuyo; 木戸, 詔子; Kido, Shoko
抄録: To clarify the formation of the vitelline membrane, we investigated changes in the proteins constituting the inner layer of the vitelline membrane during the ovum maturation in the hen ovary.The vitelline membranes were isolated from yellow yolk follicles at the different growth stages, identified as F_1 to F_8,and their protein compositions were examined by SDS-PAGE in both the presence and the absence of reducing agent. One of the major glycoproteins found in the matured ovum, GP-I, appears to exist as monomer from the early stage of the yolk deposition (F_1). The other glycoprotein, GP-II, appears as protomer of its precursor with higher molecular weight at slightly later stage (F_3) and appears to be converted into the homodimeric form at later stages (F_7~F_8). Staining the gel for the carbohydrate moiety supports this notion. The GP-I fraction prepared from the vitelline membrane of the completed eggs was separated into two components, GP-Ia and GP- Ib, based on the pH dependence in their solubility. They have similar molecular weights but different elution volumes in cation-exchange chromatography. A similar but different heterogeneity in the GP- I fraction prepared from the follicle vitelline membrane was identified.These results indicate that proteins constituting the vitelline membrane, at least GP-I and GP-II, are processed to a great extent during the ovum maturation as well as after the ovulation.2000-12-09T15:00:00Z高速液体クロマトグラフィーによる食品中の残留農薬の分析 : Sep-Pakカートリッジによるクリーンアップ処理の検討安部, 尚子Abe, Naoko近藤, 陽太郎Kondo, Yôtarohttp://hdl.handle.net/11173/13922014-03-26T01:38:50Z2000-12-09T15:00:00Zタイトル: 高速液体クロマトグラフィーによる食品中の残留農薬の分析 : Sep-Pakカートリッジによるクリーンアップ処理の検討
著者: 安部, 尚子; Abe, Naoko; 近藤, 陽太郎; Kondo, Yôtaro
抄録: It is important for analysis of the pesticide residues in vegetables to remove the UV- sensitive impurities derived from vegetables. To evaluate clean-up methods for determination of the pesticide residues, organophosphorus pesticides were spiked into the solution obtained after extraction of the vegitable samples with acetone followed by partition with dichloromethane. A portion of the dichloromethane extract was loaded onto a Sep-Pak cartrige (the cartrige is filled with C_<18>, frorisil, silica and diol material, respectively). Average recoveries from vegitable extracts were in the range 74. 3-96. 7% and the background was greatly reduced when both C_<18> and silica columns were used.2000-12-09T15:00:00Zマウスリンパ組織のレプチンレセプター発現細胞の検出阪口, 恵子Sakaguchi, Keiko佐藤, 紘子Satoh, Hiroko徳永, 雅美Tokunaga, Masami西津, 景子Nishitsu, Keiko久湊, 尚子Hisaminato, Shyoko松永, 美沙子Matsunaga, Misako宮田, 堅司Miyata, Kenjihttp://hdl.handle.net/11173/13892014-03-21T16:35:31Z2000-12-09T15:00:00Zタイトル: マウスリンパ組織のレプチンレセプター発現細胞の検出
著者: 阪口, 恵子; Sakaguchi, Keiko; 佐藤, 紘子; Satoh, Hiroko; 徳永, 雅美; Tokunaga, Masami; 西津, 景子; Nishitsu, Keiko; 久湊, 尚子; Hisaminato, Shyoko; 松永, 美沙子; Matsunaga, Misako; 宮田, 堅司; Miyata, Kenji
抄録: Leptin, the product of the ob gene expressed in adipocytes, is shown to influence energy intake and expenditure, proliferation of CD^<4+> T cells, neovascularization and intracellular triglycerides homeostasis in non-adipocytes. Leptin acts on target cells through receptor (OB-R). There are at least five different types of OB-R in mouse due to alternative splicing from db gene transcripts.OB-Ra〜OB-Rd share identical extracellular and transmembrane domains and JAK binding consensus sequence at cytoplasmic domain. Only OB-Rb has an additional STAT binding motif and is essential for most of leptin' s physiological functions through JAK-STAT pathway. OB-Ra is also reported to transduct weakly leptin's signal through JAK-phospholyration pathway. On this paper we examined which kinds of cell express OB-Ra or OB-Rb in lymphoid and fat tissues of the mouse. Both types of OB-R were detected in thymus, spleen and gastrolienal fat tissue by RTPCR method, then the constitutive cells were separated from dissected tissues and cultured in GIT medium with 10% heat-inactivated FBS. Primary cultured lymphocytes isolated from thymus or spleen expressed both OB-Ra and OB-Rb. On the other hand, in adhesive cells dispersed with enzymatic digestion and primary cultured OB-Rb was not detected, though OB-Ra detectable.Similarly, primary cultured adhesive cells of gastrolienal fat tissue expressed only OB-Ra. It is therefore most parsimonious to conclude that only lymphocytes express OB-Rb and response effectively to leptin in thymus.2000-12-09T15:00:00Z表紙ほかhttp://hdl.handle.net/11173/13902014-03-21T16:35:31Z2000-12-09T15:00:00Zタイトル: 表紙ほか2000-12-09T15:00:00Z